Rederivation of transgenic mice from iPS cells derived from frozen tissue
نویسندگان
چکیده
منابع مشابه
Cytotoxicity of Chitosan Derived from Shrimp for Bone Scaffold on Adipose Tissue-Derived Mesenchymal Stem Cells
متن کامل
Mice cloned from white adipose tissue-derived cells.
Isolation of different cells from adipose tissue was performed according to the previous reports (Rodeheffer et al., 2008; Sugii et al., 2010). Briefly, the inguinal fat pads of adult male B6D2F1 (8-12 weeks of age) mice were harvested, washed several times with phosphate-buffered saline (PBS) and excised into small pieces. The tissues were digested with 0.1% type I collagenase (Sigma) for 50 m...
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متن کاملP-118: Developmental Competence of Ovine Embryos Derived from Frozen Oocytes Co-Cultured with Embryos Derived from Fresh Oocytes
Background: Currently, a limited number of studies have been done on the vitrification of small ruminant oocytes, especially in sheep, where poor developmental rates are obtained following immature and mature oocyte vitrification. Poor ovine oocyte cryopreservation could attribute to the damage to enzymes which has role in metabolic pathways. Therefore, production of cytokines and growth factor...
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ژورنال
عنوان ژورنال: Transgenic Research
سال: 2010
ISSN: 0962-8819,1573-9368
DOI: 10.1007/s11248-010-9390-9